Development of morphological changes and ileal glucagon gene expression in the small intestine of lambs infected with Trichostrongylus colubriformis
Identifieur interne : 00D265 ( Main/Exploration ); précédent : 00D264; suivant : 00D266Development of morphological changes and ileal glucagon gene expression in the small intestine of lambs infected with Trichostrongylus colubriformis
Auteurs : E. A. Roy [Australie] ; H. Hoste [France] ; P. Fuller [Australie] ; L. Tatarczuch [Australie] ; I. Beveridge [Australie]Source :
- Journal of Comparative Pathology [ 0021-9975 ] ; 1996.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Parasitologie.
English descriptors
- KwdEn :
- Adaptive, Adaptive response, American journal, Animal, Ascaris suum, Authentic glucagon, Brush border, Cell proliferation, Chronological evolution, Colubriformis, Colubriformis infection, Comparative pathology, Control animals, Control sheep, Crypt, Crypt depth, Crypt hyperplasia, Cytological, Cytological changes, Cytological lesions, Cytological modifications, Cytopathology, Direct contact, Distal part, Distal parts, Distal response, Electron microscopy, Enteroglucagon, Enteroglucagon gene expression, Experimental disease, Experimental parasitology, Gene expression, Glucagon, Glucagon gene expression, Histological, Histological changes, Histopathology, Hoste, Ileal, Ileal glucagon gene expression, Ileal proglucagon, Ileum, Infection, Intense vacuolation, Intercellular spaces, Intestinal, Intestinal adaptation, Intestinal epithelium, Intestinal pathology, Intestinal resection, Intestine, Main site, Major role, Morphological changes, Mrna, Mucosal, Mucosal changes, Nematode, Nematode infections, Nematode parasitism, Nippostrongylus brasiliensis, Northern blots, Parasitism, Parasitology, Plasma enteroglucagon concentrations, Prepatent period, Present study, Proglucagon, Proglucagon mrna concentrations, Proglucagon synthesis, Proximal, Proximal part, Proximal region, Resection, Sheep, Significant increase, Similar process, Small intestine, Standard deviation, Trichostrongyliasis, Trichostrongylus, Trichostrongylus colubriformis, Trichostrongylus colubriformis infection, Uranyl acetate, Villous, Villous changes, Villous height, Villous length, Villus.
- Teeft :
- Adaptive, Adaptive response, American journal, Ascaris suum, Authentic glucagon, Brush border, Cell proliferation, Chronological evolution, Colubriformis, Colubriformis infection, Comparative pathology, Control animals, Control sheep, Crypt, Crypt depth, Crypt hyperplasia, Cytological, Cytological changes, Cytological lesions, Cytological modifications, Direct contact, Distal part, Distal parts, Distal response, Electron microscopy, Enteroglucagon, Enteroglucagon gene expression, Experimental parasitology, Glucagon, Glucagon gene expression, Histological, Histological changes, Hoste, Ileal, Ileal glucagon gene expression, Ileal proglucagon, Ileum, Infection, Intense vacuolation, Intercellular spaces, Intestinal, Intestinal adaptation, Intestinal epithelium, Intestinal pathology, Intestinal resection, Intestine, Main site, Major role, Morphological changes, Mrna, Mucosal, Mucosal changes, Nematode, Nematode infections, Nematode parasitism, Nippostrongylus brasiliensis, Northern blots, Parasitism, Parasitology, Plasma enteroglucagon concentrations, Prepatent period, Present study, Proglucagon, Proglucagon mrna concentrations, Proglucagon synthesis, Proximal, Proximal part, Proximal region, Resection, Significant increase, Similar process, Small intestine, Standard deviation, Trichostrongylus, Trichostrongylus colubriformis, Trichostrongylus colubriformis infection, Uranyl acetate, Villous, Villous changes, Villous height, Villous length, Villus.
Abstract
Summary: To investigate the consequences of subclinical Trichostrongylus colubriformis infection on the intestinal mucosa and the associated changes in enteroglucagon gene expression, sheep were infected with 30000 larvae and killed 5, 10, 15 or 20 days after infection. Histological and cytological changes were examined. In the main site of infection, the upper duodenum, villous atrophy associated with crypt hyperplasia developed gradually. Cytological changes in the enterocytes appeared concurrently, characterized by a progressive reduction in brush border and in the number of ribosomes in the cytoplasm, changes in the internal structure of mitochondria, and enlargement of the intercellular spaces. Neither histological nor cytological modifications were found before day 15. At the same time, villous hypertrophy developed distally, beyond the main site of infection; this was interpreted as an adaptive response to parasitism. Enteroglucagon gene expression in the ileum was measured in parallel with the mucosal changes but did not reveal any difference between infected and control sheep. The results indicate that this gastrointestinal hormone does not have a major role in the response to nematode parasitism.
Url:
DOI: 10.1016/S0021-9975(96)80077-6
Affiliations:
- Australie, France
- Centre-Val de Loire, Région Centre, Victoria (État)
- Melbourne, Nouzilly
- Université de Melbourne
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Le document en format XML
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<term>Animal</term>
<term>Ascaris suum</term>
<term>Authentic glucagon</term>
<term>Brush border</term>
<term>Cell proliferation</term>
<term>Chronological evolution</term>
<term>Colubriformis</term>
<term>Colubriformis infection</term>
<term>Comparative pathology</term>
<term>Control animals</term>
<term>Control sheep</term>
<term>Crypt</term>
<term>Crypt depth</term>
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<term>Cytological changes</term>
<term>Cytological lesions</term>
<term>Cytological modifications</term>
<term>Cytopathology</term>
<term>Direct contact</term>
<term>Distal part</term>
<term>Distal parts</term>
<term>Distal response</term>
<term>Electron microscopy</term>
<term>Enteroglucagon</term>
<term>Enteroglucagon gene expression</term>
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<term>Experimental parasitology</term>
<term>Gene expression</term>
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<term>Glucagon gene expression</term>
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<term>Histological changes</term>
<term>Histopathology</term>
<term>Hoste</term>
<term>Ileal</term>
<term>Ileal glucagon gene expression</term>
<term>Ileal proglucagon</term>
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<term>Infection</term>
<term>Intense vacuolation</term>
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<term>Intestinal adaptation</term>
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<term>Proximal region</term>
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<term>Significant increase</term>
<term>Similar process</term>
<term>Small intestine</term>
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<term>Trichostrongyliasis</term>
<term>Trichostrongylus</term>
<term>Trichostrongylus colubriformis</term>
<term>Trichostrongylus colubriformis infection</term>
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<term>Villous changes</term>
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<term>Expression génique</term>
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<term>Iléon</term>
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<term>Crypt depth</term>
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<term>Distal parts</term>
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<term>Glucagon gene expression</term>
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<term>Ileal glucagon gene expression</term>
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<term>Infection</term>
<term>Intense vacuolation</term>
<term>Intercellular spaces</term>
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<term>Intestinal resection</term>
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<term>Major role</term>
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<term>Mucosal changes</term>
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<term>Parasitology</term>
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<term>Present study</term>
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<front><div type="abstract" xml:lang="en">Summary: To investigate the consequences of subclinical Trichostrongylus colubriformis infection on the intestinal mucosa and the associated changes in enteroglucagon gene expression, sheep were infected with 30000 larvae and killed 5, 10, 15 or 20 days after infection. Histological and cytological changes were examined. In the main site of infection, the upper duodenum, villous atrophy associated with crypt hyperplasia developed gradually. Cytological changes in the enterocytes appeared concurrently, characterized by a progressive reduction in brush border and in the number of ribosomes in the cytoplasm, changes in the internal structure of mitochondria, and enlargement of the intercellular spaces. Neither histological nor cytological modifications were found before day 15. At the same time, villous hypertrophy developed distally, beyond the main site of infection; this was interpreted as an adaptive response to parasitism. Enteroglucagon gene expression in the ileum was measured in parallel with the mucosal changes but did not reveal any difference between infected and control sheep. The results indicate that this gastrointestinal hormone does not have a major role in the response to nematode parasitism.</div>
</front>
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<affiliations><list><country><li>Australie</li>
<li>France</li>
</country>
<region><li>Centre-Val de Loire</li>
<li>Région Centre</li>
<li>Victoria (État)</li>
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<tree><country name="Australie"><region name="Victoria (État)"><name sortKey="Roy, E A" sort="Roy, E A" uniqKey="Roy E" first="E. A." last="Roy">E. A. Roy</name>
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<name sortKey="Beveridge, I" sort="Beveridge, I" uniqKey="Beveridge I" first="I." last="Beveridge">I. Beveridge</name>
<name sortKey="Fuller, P" sort="Fuller, P" uniqKey="Fuller P" first="P." last="Fuller">P. Fuller</name>
<name sortKey="Tatarczuch, L" sort="Tatarczuch, L" uniqKey="Tatarczuch L" first="L." last="Tatarczuch">L. Tatarczuch</name>
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<country name="France"><region name="Centre-Val de Loire"><name sortKey="Hoste, H" sort="Hoste, H" uniqKey="Hoste H" first="H." last="Hoste">H. Hoste</name>
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